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expresSF+®
Protein Sciences developed expresSF+® cells to better optimize production of recombinant proteins and are the cornerstone of Protein Sciences' baculovirus expression vector system for cost-effective research and for the cGMP production of recombinant proteins.
Uninfected expresSF+® |
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AcNPV Infected expresSF+® Cells |
Advantages of expresSF+ cells
- FDA Qualified
- Serum-Free - low cost growth media
- High-Density growth and gene expression capability
- Produces high titer AcNPV (108 PFU/mL)
- Infected with low MOI < 1
- Stable for > 50 passages
- 18-24 hour doubling time
- Do not clump or aggregate
- Scalable for cGMP manufacturing
FDA Qualified: The expresSF+ cell line is the first insect cell line commercially available to scientists that was derived from Master and Working Cell Banks of the parental SF9 cell line. Protein Sciences has worked with the FDA to characterize and qualify them for:
- Identity and stability
- Freedom of mycoplasma, spiroplasma, and other adventitious organism contamination
- Freedom of retrovirus-like particles and is non-tumorgenic in nude mice
Serum-Free: expresSF+ cells are free of exposure to serum, including during storage, and double about every 24 hours in commercially available serum-free media. Unlike other serum-free insect cells, expresSF+® cells also generate high titers of baculovirus. Therefore a single cell line can be used for the production of recombinant baculoviruses and proteins.
High Density: expresSF+ cells can be grown to a density of 108 cells/ml. At a density of 107 cells/ml, the cells are nearly 100% viable in standard suspension cultures. Recombinant protein production thus can be accomplished at densities of more than 107 cells/ml in a high density bioreactor process developed by Protein Sciences and optimized for expresSF+ cells.
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Exponential growth of expresSF+ cells: The growth of expresSF+ cells in a 2 liter bioreactor is exponential from 1.5X106 to more than 70X106 cells/mL in a serum free culture medium. Cell viability remains 98% or higher.
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